| 1. | Spike protein was the main protective antigen inducing the neutralization antibody . the fragment containing antigenic sites b and c locating at the 5 " terminus in spike gene of tgev was deleted in prcv
Tgevs蛋白可诱导产生中和抗体，是主要的保护性抗原，其n端抗原位点b和c在prcv中缺失。 |
| 2. | We wish to find some new secreted proteins of mtb h37rv . these new secreted proteins may be useful in the discovery of protective antigen , immune taxonomy and new medicine targets
作为探索性研究，这些新的分泌性蛋白可能会在保护性抗原、免疫分型以及寻找新的药物靶点等研究中提供一些线索。 |
| 3. | It is that recombinant plasmid which contain a protective antigen gene was rejected into animal ' s muscle , then the protein that the recombinant plasmid expressed in vivo will directly induce immune response in the body
该技术将含有外源蛋白编码序列的质粒dna注入某种动物，外源蛋白可以在体内直接表达并持续刺激机体产生免疫应答。 |
| 4. | It has been showed that hepatitis b virus ( hbv ) surface antigen pres , an important protective antigen , possesses many biological functions , and thus is considered to be closely associated with establishment of hbv infection , hbv replication and assembly in hepatocytes
Hbv表面抗原前s区具有丰富的生物学功能和较强的免疫原性，前s区与hbv对肝细胞的感染、病毒复制及病毒颗粒的装配密切相关，是hbv重要的保护性抗原。 |
| 5. | Css is the one of the colonization factor antigens which is a protective antigen can cause immune reaction by the means taking orally . in this study , carrot was separately transformed with agrobacterium tumefacience strain lba4404 that contains ctb or ctb - cs3 fused gene in order to get oral diarrhea vaccine . this is a potent strategy to produce etec oral vaccine
本研究期望通过农杆菌转化系统将ctb (霍乱毒素b亚基，可作为佐剂和载体)基因和ctb - cs3融合基因分别转入胡萝卜植株，使ctb和ctb ? cs3融合基因稳定整合到胡萝卜基因组内，希望以此获得以胡萝卜为受体的etec口服疫苗，使服食者在进食的同时就可获得腹泻免疫。 |
| 6. | The results demonstrated that the momps were protective antigens and the momp - iscoms of aeromonas hydrophila could induce the host to mount satisfied immunity . a pair of primers were designed according to the published nucleotide sequence of a putative outer membrane protein gene ( omp ) of aeromonas hydrophila . with the specific primers , a target fragment about 1 . 1kb was amplified from aeromonas hydrophila l316 via pcr . the target fragment was inserted into the linearized pgem - t easy vector
根据已发表嗜水气单胞菌的外膜蛋白基因omp的核苷酸序列设计引物，利用pcr技术，扩增、克隆了嗜水气单胞菌l316的主要外膜蛋白基因( momp ) ，经t a克隆，插入到pgem - t系列载体上，测序分析结果表明momp基因最长的开放阅读框( orf )为1035nt ，编码由344个氨基酸组成，分子量为36kda的主要外膜蛋白质( momp ) 。 |
| 7. | A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv
以猪瘟兔化弱毒犊牛睾丸细胞毒( hclv )中提取的细胞总rna为模板，以rt - pcr技术，扩增出了完整e2基因的cdna片段。经电泳、酶切分析，证实了所扩增片段为e2基因特异性片段。 |
| 8. | Classical swine fever ( csf ) is one of major contagious diseases in pig breeding industry . in order to gain massive diagnostic antigen csfv , and large scale of csfv specific major protective antigen protein ( e2 envelope glycoprotein ) in eucaryotic cells , which are prepared into subunit vaccine for effective prevention against csf
猪瘟是危害养猪业的主要传染病，为了得到大量的猪瘟病毒特异性诊断抗原，并试图在真核细胞中大量表达猪瘟病毒特有的免疫保护性抗原，即e2囊膜糖蛋白，为进一步制备猪瘟亚单位疫苗有效预防本病奠定基础。 |
| 9. | These essential component , hemagglutination , toxicity , immunogenicity of mycoplasma hyopneumoniae strain xy - 1 membrane protein were studied . the range of membrane protein mw is from 14kd to 100kd by means of sodium dodecyl sulfate - polyacrylamide gel electrophoresis , and molecular weights of the protective antigens were 36kd and 64kd by sds - page
本研究以分离猪肺炎支原体野毒株( xy - 1株)的膜蛋白为试验材料，系统地研究了膜蛋白的分子量范围、血凝性、毒性及免疫原性等。 |
